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Postdoctoral Researcher – Molecular Drivers of BRCA1-Related Precancer

Antoni van Leeuwenhoek -
Amsterdam

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Vacaturegegevens

Fulltime
€ 3.903 - € 5.503 per maand
14 uur geleden

Secundaire arbeidsvoorwaarden

Kosteloos parkeren
Reiskostenvergoeding
Budget voor professionele ontwikkeling

Kwalificaties

Biologie
Doctor
Cellenbiologie
Analytisch vermogen

Volledige vacaturetekst

We are recruiting a postdoctoral researcher to join a newly funded Gray Foundation Team Science project tackling one of the most important unsolved problems in cancer prevention: what drives the very first steps of breast cancer initiation in BRCA1 mutation carriers. Working at the interface of human tissue biology, single-cell genomics, spatial imaging, and functional mouse modeling, you will help map the genetic and phenotypic landscape of BRCA1-deficient precancerous cells in prophylactic mastectomy specimens and translate those discoveries into functionally validated driver candidates – a unique opportunity to do science that directly connects to clinical cancer interception.

The Jonkers laboratory at the Netherlands Cancer Institute has built a world-leading platform for functional breast cancer modeling, combining germline genetics, intraductal somatic engineering, and quantitative in vivo imaging. We are now applying that platform to study the biology of BRCA1-associated precancer and are looking for an exceptional postdoctoral researcher to lead this work.

You will work with a novel mouse model that combines germline heterozygous Brca1 deficiency with a dual-color fluorescent reporter that marks cells undergoing Cre-mediated loss of heterozygosity (LOH) in real time. Our preliminary data show that Brca1 LOH cells are actively cleared from the ductal epithelium in vivo, and this clearance is rescued by Trp53 loss. This provides a quantitative and mechanistic benchmark for ranking candidate perturbations.

Candidate driver genes will be introduced somatically at low frequency in mice – closely resembling the occurrence of early mutations in BRCA1 mutation carriers – through intraductal delivery of high-titer lentiviruses encoding Cre with cDNAs for overexpression or CRISPR guides for loss-of-function. Candidates will be prioritized from human precancer genomic data and from in vitro functional genetic screens. The readout will be whole-mount imaging of entire mammary glands of our mouse model, at single-cell resolution. This enables quantification of clone persistence, expansion, and progression to early lesion formation across the full gland. In parallel, you will investigate how immune-epithelial interactions in precancerous ducts impact clonal persistence and tumorigenesis.

Why the Netherlands Cancer Institute?

Science is at the heart of everything we do at the NKI. But breakthroughs don't happen through science alone; they happen through people – curious minds, bold thinkers, people who challenge assumptions and turn complex questions into meaningful discoveries. Around 600 scientists, together with 150 scientific and 150 administrative professionals, work here across the full spectrum of cancer research, from fundamental science to clinical innovation. With roughly 55 nationalities represented, we form a vibrant international community where English is our shared language and every voice carries equal weight. Our institute sits just 15 minutes by bike from the heart of Amsterdam.

You will be working at the intersection of genetics, cell biology, and cancer immunology, within a collaborative multi-laboratory environment. The ability to communicate across those boundaries, and to take ownership of a project while contributing generously to those around you, is essential. Furthermore:

You hold a PhD in Cancer Biology, Cell Biology, Molecular Biology, or a closely related discipline
You are a rigorous, analytical, and quantitative scientist with a genuine interest in mechanistic in vivo research
Experience with mouse models, lentiviral systems, CRISPR, or mammary gland biology is an advantage
Intellectual curiosity and experimental precision matter more than experience with any specific technique
You think critically about experimental setups and how to address scientific questions effectively
You enjoy reading across disciplines and integrating insights from different fields
You are comfortable with the iterative nature of in vivo research

Your development opportunities and employment conditions

The basis for your employment conditions is in accordance with the CLA Hospitals. You will receive from us:

A contract for three years for 36 hours per week;
A gross monthly salary between € 3.903,- and € 5.503,-, based on a 36-hour working week, in line with FWG 55 and depending on your experience;
144 holiday hours and 57 hours of Personal Life Budget, with full-time employment;
Annual holiday pay of 8.33% and a fixed end-of-year bonus of 8.33%;
A travel allowance of €0.23 per km;
Free parking at the Netherlands Cancer Institute. And with our bicycle plan and discount on public transport we also make it attractive for you to leave the car at home;
A great opportunity in a specialised hospital where you can also continue to learn and grow yourself if you wish: the AVL Academy offers innovative and inspiring education in the field of oncology professional knowledge, skills and personal development;
Opportunity to exercise at our Fit Boutiqs;
An active staff association.

Interested?

We are curious about your talent! Will you be the one to make a difference in the world of cancer care and research? Apply now by using the apply button. Please attach your CV, a brief cover letter explaining why this project excites you and what would you bring to it + the names of two references to the application form. Make sure to include your reference information together with your CV or cover letter, as there is no option to submit them separately.

For further information about the position, please contact Jos Jonkers (Senior groupleader) via [email protected]. For questions about the application procedure, please contact Daphne Heemskerk (recruiter) via [email protected].

Review of applications begins immediately. The position is open until filled.

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